Aquaculture Research，2008,39(3)：240-251

Five diets that contained fresh squid meat as the basic constituent and were supplemented with different amounts of highly unsaturated fatty acids (HUFA) and astaxanthin were fed to pond-reared Penaeus monodon broodstock. Diet A was sole squid meat. Diets B and C were supplemented with astaxanthin 50 and 100 mg kg−1 respectively. Diets D and E were supplemented with HUFA 5 and 10 g.kg−1 and astaxanthin 50 mg kg−1 respectively. The result showed that the group fed diet E had the best reproductive performance in all experimental groups. It had a higher proportion of spawns (71.5%), spawning rate (0.047), a shorter latency period (7.7±0.3 d), higher absolute fecundity (× 103) (361.6±5.5) and egg production/female (× 103) (597.0±18.0) than all the other experimental groups. The fatty acid composition in broodstock diets strongly affected the tissue and fecundity of broodstock. Good correlations between the content of 20:4n-6 in eggs and the fecundity (r2=0.6109) and egg production (r2=0.9876) of broodstock were found. On the other hand, 22:6n-3 and DHA/EPA ratio was negatively correlated with the fecundity of broodstock (r2=0.5362, 0.8702 respectively). The result also showed that the balance between n-3 and n-6 fatty acid families, total polyunsaturated fatty acids and total saturated fatty acid and 20:5n-3 (EPA) and 22:6n-3 (DHA) may play vital roles in maturation and reproductive performance of P. monodon broodstock.

黄建华，江世贵, 林黑着，周发林，叶 乐